RNA Synthesis
The RNA Medicine Core offers both reporter mRNAs and customizable RNA synthesis services. Our reporter gene mRNAs include EGFP, EYFP, mCherry, FFLuc, Renilla Luc, and Nano Luc. We provide 100 up to 15 mg of Cap-1 mRNAs and mRNAs with 5-methoxyuridine (5moU) and N1-methylpseudouridine (N1MePsU) modification. These mRNAs are capped and polyadenylated, making them ready for transfection and expression in vitro (e.g. < 100 ug) or in vivo (e.g. > 300 ug), providing flexibility and scalability for your diverse research needs both in vitro and in vivo.
What We Offer
Catalog | Gene | Capping and modification | Quantity | Storage and Buffer | Timeline (Business days) |
|---|---|---|---|---|---|
mRNA | Reporter gene | Cap-1 mRNA (5moU) | 100 ug to 15 mg | −80 °C, RNase free water (default)/Client Spec | 1-5 |
mRNA | Reporter gene | Cap-1 mRNA (N1MePsU) | 100 ug to 15 mg | −80 °C, RNase free water (default)/Client Spec | 1-5 |
mRNA | Reporter gene | Cap-1 mRNA | 100 ug to 15 mg | −80 °C, RNase free water (default)/Client Spec | 1-5 |
Custom RNA | Custom gene | Custom lncRNA | Client Spec | −80 °C, RNase free water (default)/Client Spec | 1-2 weeks |
Custom RNA | Custom gene | Custom Cap-1 mRNA (N1MePsU) | Client Spec | −80 °C, RNase free water (default)/Client Spec | 1-2 weeks |
Custom RNA | Custom gene | Custom Cap-1 mRNA (5moU) | Client Spec | −80 °C, RNase free water (default)/Client Spec | 1-2 weeks |
RNA Portfolio
EGFP mRNA
The EGFP mRNA produces a green fluorescent protein (26.94kDa) with an excitation peak at 488 nm and an emission peak at 509 nm.
EYFP mRNA
The EYFP mRNA produces yellow-green fluorescent protein(27.07kDa) with an excitation peak at 516 nm and an emission peak at 527 nm.
mCherry mRNA
The mCherry mRNA produces a red fluorescent protein (26.72kDa) with an excitation peak at 587 nm and an emission peak at 610 nm.
FFLuc mRNA
Firefly Luciferase (FFluc, 60.64kDa) uses D-Luciferin as substrate and emits light in the yellow-green spectrum, peaks ~560 nm.
Renilla Luc mRNA
Renilla luciferase (Rluc, 35.99kDa) uses coelenterazine as its substrate and emits light in the blue-green spectrum, peaks ~480 nm.
NanoLuc mRNA
NanoLuc luciferase (Nluc, 19.09kDa) uses furimazine as substrate, which produces high-intensity, glow-type luminescence. NanoLuc generates brighter signal compared to Firefly and Renilla luciferases.
OVA mRNA
Ovalbumin (OVA, 45kDa) is a protein often used as a model antigen in immunological research. It helps scientists study immune responses and develop vaccines.
PD-L1 mRNA
Programmed death-ligand 1 (PD-L1), a type I transmembrane protein (~ 40 kDa), plays a key role in immune regulation by binding to the PD-1 receptor on T cells to inhibit immune responses.
IL2RB mRNA
Interleukin-2 receptor subunit beta (IL2RB), a ~40 kDa type I membrane glycoprotein, mediates IL-2/IL-15 signaling and has been explored as a vaccine target to enhance T-cell activation and immune responses.
Under development
Anti-CTLA-4 mRNA
Quality RNA at Every Scale
The listed RNAs are intended for research purposes only. Both internal UHN users and external users are kindly requested to contact us for further information.
Catalog | Scale 1 | Scale 2 | Scale 3 | Scale 4 | Scale 5 |
|---|---|---|---|---|---|
100 ug | 300 ug | 500 ug | 1 mg | >= 5 mg | |
Custom RNA (Cap-1/5moU/N1MePsU) | Contact us | Contact us | Contact us | Contact us | Contact us |
Reporter mRNA (5moU/N1MePsU) | Contact us | Contact us | Contact us | Contact us | Contact us |
Reporter mRNA | Contact us | Contact us | Contact us | Contact us | Contact us |
Quality-Driven Potency Testing
RNA Quality Control
QC Category | QC | Method | Regular Test | Add on |
|---|---|---|---|---|
Identification | Buffer Specification | Client Spec | Yes | No |
Impurity | RNA Integrity/size | Gel electrophoresis at 200ng | Yes | No |
Identification | RNA Concentration | UV Absorbance by Nanodrop | Yes | No |
Identification
| Appearance | Visual Inspection | Yes | No |
Bioluminescence Assays
For detecting Firefly Luciferase (FFLuc), Renilla Luciferase (Rluc), and NanoLuc Luciferase (Nluc) in mRNA bioluminescence assays, we recommend using these kits:
1. Firefly Luciferase (FFLuc):
ONE-Glo® Luciferase Assay System: This kit allows highly sensitive, robust detection of firefly luciferase reporter gene expression in mammalian cells. It's widely used for its sensitivity and ease of use.
2. Renilla Luciferase (Rluc):
Renilla-Glo® Luciferase Assay System: This system provides a single-addition reagent that generates a glow-type signal in the presence of Renilla luciferase. It's known for its high sensitivity and stability.
3.NanoLuc Luciferase (Nluc):
Nano-Glo® Luciferase Assay System: This kit is specifically designed for NanoLuc luciferase detection. It offers a bright, long-lasting signal and is highly sensitive, making it suitable for applications where sensitivity is critical.