Some technical information on the specific LNPs that the RNA CORE offers?

The pharmacology, the chemistry and the formulation of the LNPs:

1. The chemistry and the formulation of the LNPs

2. Bio distribution

3. Half life

4. Clearance mechanisms

5. Toxicity

6. mRNA/siRNA cargo capacity

7. Whose formulation is this?

8. Reference?

More pharmacology Information.

We advise customers to test various concentrations of siRNA/mRNA on their own. As a starting point, we suggest using siRNA-LNP at a final siRNA concentration of 100 nM and mRNA-LNP at 1 ng/µL of mRNA for in vitro experiment.

siRNA-LNPs: 2 mg/kg, i.v. injection, 1 injection every 3 days for 2-week period to 3 weeks period. Reporter mRNA-LNP: 0.2 mg/kg. i.v. injection, 1 injection only, 24h post i.v. imaging test.

We suggest using cell viability(>80%) to determine the optimal time points for assessing gene expression.

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On-shelf reporter mRNA-LNP requires one working day plus shipping time, while custom RNA-LNPs typically takes 1 week, depending on the manufacturing scale.

For our standard LNP formulation, you only need to provide siRNA (>= 3 nmol) / mRNA (>= 50 ug) to us. For customized LNP formulation, please contact us.

Lipofectamine™ RNAiMAX Transfection Reagent

It is generally not necessary when using lipid nanoparticles. The LNPs are designed to facilitate the entry of RNA into cells without the need for additional methods like electroporation.

We provide reporter mRNAs (EGFP, EYFP, and mCherry). After treatment, use fluorescence-activated cell sorting (FACS) to isolate the cells that have taken up the labeled RNA-LNP.

The RNA-LNP we provide is sterile and ready to use for both in vitro and in vivo applications. For standard intracellular transfection, simply co-culture the RNA-LNP with cells in complete medium at 37 °C and 5% CO₂ for 24 hours. For specialized applications, we do not provide a specific treatment protocol; users should optimize their own methods according to their experimental requirements.

For the RNA-LNP Kit, the LNP is made using manual pipetting, which may result in variations in LNP quality. For our formal RNA-LNP service, we use a microfluidic device and flow control system to prepare the LNP, followed by post-purification and QC tests using various instruments.

All procedures for cell transfection using our quick RNA-LNP Kits are detailed and have been successfully tested with our reporter gene mRNA. The potency and case test results confirm the method's effectiveness.

Add the RNA-LNP complex to cells and incubate for 24h at 37°C and 5% CO2. The lipid nanoparticles will fuse with the cell membrane, allowing the RNA to enter the cells.