We advise customers to test various concentrations of siRNA/mRNA on their own. As a starting point, we suggest using siRNA-LNP at a final siRNA concentration of 100 nM and mRNA-LNP at 1 ng/µL of mRNA for in vitro experiment.

siRNA-LNPs: 2 mg/kg, i.v. injection, 1 injection every 3 days for 2-week period to 3 weeks period. Reporter mRNA-LNP: 0.2 mg/kg. i.v. injection, 1 injection only, 24h post i.v. imaging test.

We suggest using cell viability(>80%) to determine the optimal time points for assessing gene expression.

On-shelf reporter mRNA-LNP requires one working day plus shipping time, while custom RNA-LNPs typically takes 1 week, depending on the manufacturing scale.

siRNA-LNP: 4 °C, 3 months, sterile, open inside BSC;

mRNA-LNP: -80 °C, 3 months, avoid multiple freeze thaw cycles

For our standard LNP formulation, you only need to provide siRNA (>= 3 nmol) / mRNA (>= 50 ug) to us. For customized LNP formulation, please contact us.

Lipofectamine™ RNAiMAX Transfection Reagent

It is generally not necessary when using lipid nanoparticles. The LNPs are designed to facilitate the entry of RNA into cells without the need for additional methods like electroporation.

We provide reporter mRNAs (EGFP, EYFP, and mCherry). After treatment, use fluorescence-activated cell sorting (FACS) to isolate the cells that have taken up the labeled RNA-LNP.

The RNA-LNP we provide is sterile and ready to use for both in vitro and in vivo applications. For standard intracellular transfection, simply co-culture the RNA-LNP with cells in complete medium at 37 °C and 5% CO₂ for 24 hours. For specialized applications, we do not provide a specific treatment protocol; users should optimize their own methods according to their experimental requirements.

Add the RNA-LNP complex to cells and incubate for 24h at 37°C and 5% CO2. The lipid nanoparticles will fuse with the cell membrane, allowing the RNA to enter the cells.