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Ionizable Lipid Synthesis

Ionizable Lipid Synthesis involves the production of custom lipids for the formulation of lipid nanoparticles (LNPs), which are used to deliver nucleic acids to target cells. Different synthesis schemes designed for different moiety or functional group in a molecule, and the synthesis may involve a dedicated equipment to improve the final product purity and the reaction yield. Our specialized equipment will boost your molecule design, and they include: OMNI-Lab Glovebox system for different reaction; flash chromatography and rotary evaporator system for raw material or precursor purification; preparative or semi-preparative HPLC system for high purity final products. 

OMNI-Lab Glovebox system

The OMNI-Lab glovebox system provides a sealed container to allow for the manipulation of objects in a highly pure inert atmosphere. The inert gas (nitrogen) in a glovebox is pumped through a series of treatment devices which remove solvents, water and oxygen from the gas. In addition, this system is equipped with integrated purifier, controls, display and stand for real-time monitor and control of pressure and moisture level.

 

Specifications and Features:

  • Gas purification system capacity: 20 L of oxygen and 2.3 kg of water absorption

  • Menu driven interface controlled via an operator touch screen graphical display

  • Three adjustable shelves

  • Built-in programmed control of purifier regeneration and lab pressure

  • Built in system diagnostics

Lipid Purification

(coming soon!)

Flash chromatography with an evaporative light-scattering detector (ELSD) is a powerful tool for lipid purification because it allows for rapid, efficient separation of lipids from other biomolecules in a sample. It is often used in lipid research and other fields where purification of complex mixtures is required. The facility provide with a BUCHI Pure C-815 Flash system for lipids product purification.

 

Specifications and Features:

  • Flow rate (flash mode): 250 ml/min

  • Pressure range (flash mode): 0 – 50 bar

  • UV - Vis wavelength range: 200 ─ 800 nm

  • Detector: ELSD

RV 10 auto pro V-C Complete

(coming soon!)

A rotary evaporator can be useful in a lipid synthesis processing for removing solvents or other volatile components from lipid solutions. In addition to the rotary evaporator RV 10 auto, the package includes the heating bath HB digital with a capacity of 4 liters, the vacuum pump VACSTAR digital and the recirculating chiller RC 2 lite. All components and connections required for operation are included.

Waters Prep 150 HPLC system with UV/Vis detector

The Prep 150 HPLC System is the perfect fit-for-purpose solution for the rapid isolation and collection of your valuable complex samples with complete confidence. This system allows for sample purification and scale methods from analytical to prep. In the facility, the Prep 150 HPLC system is equipped with a 2489 UV/Vis detector, which is the most sensitive and versatile dual-wavelength absorbance detector available for HPLC, with a design that combines a high energy Deuterium source, a precise optical design, and low-noise, high-speed electronics.

Specifications and Features:

  • Mobile phase mixing: binary or quaternary

  • Flow rates: up to 150 mL/min.

  • Sample injection mode: manual or automated

  • Purification capacity:  Sample loads of milligrams to grams (with up to 50 mm column I.D.)

  • Software: ChromScope

  • UV/Vis linear range of 2.5 AU enables the identification and quantitation of both major and minor components in the same run

  • Dual wavelength montioring

  • Sampling rate: 1 to 80 Hz

Waters semi-prep HPLC system with PDA detector

The Waters semi-prep HPLC system is ideal for purifying samples from micrograms to milligrams. Equipped with a highly sensitive 2998 PDA detector and a 2767 sample manager that can collect large amounts of sample, this system provides a high throughput way for rapid sample purification.

Specifications and Features:

  • 2D and 3D spectral analysis

  • High spectral resolution: 1.2 nm

  • Flexible sampling rates: 1 to 80 Hz

  • Sample collection capacity: up to 15 microtiter plates or 580 test tubes

  • Automated sample injection.

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